Expanding the toolbox for cryopreservation of marine and freshwater diatoms.
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De Decker, Sam
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Diatoms constitute the most diverse group of microalgae and have long been recognised for their large biotechnological potential. In the wake of growing research interest in new model species and development of commercial applications, there is a pressing need for long-term preservation of diatom strains. While cryopreservation using dimethylsulfoxide (DMSO) as a cryoprotective agent is the preferred method for long-term strain preservation, many diatom species cannot be successfully cryopreserved using DMSO. Therefore, in this study, we studied cryopreservation success in six different diatom species, representing the major morphological and ecological diatom groups, using a range of DMSO concentrations and Plant Vitrification Solution 2 (PVS2) as an alternative cryoprotectant to DMSO. In addition, we tested whether suppressing bacterial growth by antibiotics accelerates the postthaw recovery process. Our results show that the effects of cryoprotectant choice, its concentrati.....
Sustainable Development Goals (SDG)14.a
Essential Ocean Variables (EOV)N/A
DOI Original10.1038/s41598-018-22460-0 1
CitationStock, W., Pinseel, E., De Decker, S. et al. (2018) Expanding the toolbox for cryopreservation of marine and freshwater diatoms. Scientific Reports, 8:4279, 9pp. DOI. https://doi.org/10.1038/s41598-018-22460-0
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