dc.contributor.author | Stock, Willem | |
dc.contributor.author | Pinseel, Eveline | |
dc.contributor.author | De Decker, Sam | |
dc.contributor.author | Sefbom, Josefin | |
dc.contributor.author | Blommaert, Lander | |
dc.contributor.author | Chepurnova, Olga | |
dc.contributor.author | Sabbe, Koen | |
dc.contributor.author | Vyverman, Wim | |
dc.date.accessioned | 2021-07-05T18:00:02Z | |
dc.date.available | 2021-07-05T18:00:02Z | |
dc.date.issued | 2018 | |
dc.identifier.citation | Stock, W., Pinseel, E., De Decker, S. et al. (2018) Expanding the toolbox for cryopreservation of marine and freshwater diatoms. Scientific Reports, 8:4279, 9pp. DOI. https://doi.org/10.1038/s41598-018-22460-0 | en_US |
dc.identifier.uri | https://repository.oceanbestpractices.org/handle/11329/1610 | |
dc.identifier.uri | http://dx.doi.org/10.25607/OBP-1321 | |
dc.description.abstract | Diatoms constitute the most diverse group of microalgae and have long been recognised for their
large biotechnological potential. In the wake of growing research interest in new model species and
development of commercial applications, there is a pressing need for long-term preservation of
diatom strains. While cryopreservation using dimethylsulfoxide (DMSO) as a cryoprotective agent is
the preferred method for long-term strain preservation, many diatom species cannot be successfully
cryopreserved using DMSO. Therefore, in this study, we studied cryopreservation success in six different
diatom species, representing the major morphological and ecological diatom groups, using a range
of DMSO concentrations and Plant Vitrification Solution 2 (PVS2) as an alternative cryoprotectant to
DMSO. In addition, we tested whether suppressing bacterial growth by antibiotics accelerates the postthaw
recovery process. Our results show that the effects of cryoprotectant choice, its concentration
and the addition of antibiotics are highly species specific. In addition, we showed that PVS2 and
antibiotics are useful agents to optimize cryopreservation of algae that cannot survive the traditional
cryopreservation protocol using DMSO. We conclude that a species-specific approach will remain
necessary to develop protocols for diatom cryopreservation and to increase their representation in
public culture collections. | en_US |
dc.language.iso | en | en_US |
dc.rights | Attribution 4.0 | * |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | * |
dc.subject.other | Diatoms | en_US |
dc.subject.other | Microalgae | en_US |
dc.subject.other | Cryopreservation | |
dc.title | Expanding the toolbox for cryopreservation of marine and freshwater diatoms. | en_US |
dc.type | Journal Contribution | en_US |
dc.format.pagerange | 9pp. | en_US |
dc.identifier.doi | 10.1038/s41598-018-22460-0 1 | |
dc.bibliographicCitation.title | Scientific Reports | en_US |
dc.bibliographicCitation.volume | 8 | en_US |
dc.bibliographicCitation.issue | Article 4279 | en_US |
dc.description.sdg | 14.a | en_US |
dc.description.eov | N/A | en_US |
dc.description.adoption | Validated (tested by third parties) | en_US |
dc.description.methodologyType | Method | en_US |
dc.description.methodologyType | Reports with methodological relevance | en_US |
obps.contact.contactname | Wim Vyverman | |
obps.resourceurl.publisher | https://www.nature.com/articles/s41598-018-22460-0 | |