Modified FlowCAM procedure for quantifying size distribution of zooplankton with sample recycling capacity.
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Sastri, Akash R.
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We have developed a modified FlowCAM procedure for efficiently quantifying the size distribution of zooplankton. The modified method offers the following new features: 1) prevents animals from settling and clogging with constant bubbling in the sample container; 2) prevents damage to sample animals and facilitates recycling by replacing the built-in peristaltic pump with an external syringe pump, in order to generate negative pressure, creates a steady flow by drawing air from the receiving conical flask (i.e. vacuum pump), and transfers plankton from the sample container toward the main flowcell of the imaging system and finally into the receiving flask; 3) aligns samples in advance of imaging and prevents clogging with an additional flowcell placed ahead of the main flowcell. These modifications were designed to overcome the difficulties applying the standard FlowCAM procedure to studies where the number of individuals per sample is small, and since the FlowCAM can only ima.....
Issue4 ( e0175235)
Sustainable Development Goals (SDG)14.a
Maturity LevelPilot or Demonstrated
DOI Originalhttps://doi.org/10.1371/journal. pone.0175235
CitationWong, E., Sastri, A.R., Lin, F-S. and Hsieh, C-h (2017) Modified FlowCAM procedure for quantifying size distribution of zooplankton with sample recycling capacity. PLoS ONE ,12(4): e0175235, 12pp. DOI: https://doi.org/10.1371/journal. pone.0175235
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