Comparison of Two 16S rRNA Primers (V3–V4 and V4–V5) for Studies of Arctic Microbial.
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Cardozo-Mino, Magda G.
Rapp, Josephine Z.
Tegetmeyer, Halina E.
Buttigieg, Pier Luigi
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Microbial communities of the Arctic Ocean are poorly characterized in comparison to other aquatic environments as to their horizontal, vertical, and temporal turnover. Yet, recent studies showed that the Arctic marine ecosystem harbors unique microbial community members that are adapted to harsh environmental conditions, such as near-freezing temperatures and extreme seasonality. The gene for the small ribosomal subunit (16S rRNA) is commonly used to study the taxonomic composition of microbial communities in their natural environment. Several primer sets for this marker gene have been extensively tested across various sample sets, but these typically originated from low-latitude environments. An explicit evaluation of primer-set performances in representing the microbial communities of the Arctic Ocean is currently lacking. To select a suitable primer set for studying microbiomes of various Arctic marine habitats (sea ice, surface water, marine snow, deep ocean basin, and d.....
JournalFrontiers in Microbiology
Sustainable Development Goals (SDG)14.a
Essential Ocean Variables (EOV)Microbe biomass and diversity
Spatial CoverageArctic Ocean
CitationFadeev, E., Cardozo-Mino, M.G., Rapp, J.Z., Bienhold, C., Salter, I., Salman-Carvalho, V., Molari, M., Tegetmeyer, H.E., Buttigieg, P.L. and Boetius, A. (2021) Comparison of Two 16S rRNA Primers (V3–V4 and V4–V5) for Studies of Arctic Microbial Communities. Frontiers in Microbiology, 12:637526, 11pp. DOI: 10.3389/fmicb.2021.637526
- Arctic Practices 
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