dc.contributor.author | Boatman, Tobias G. | |
dc.contributor.author | Geider, Richard J. | |
dc.contributor.author | Oxborough, Kevin | |
dc.date.accessioned | 2020-05-28T16:43:28Z | |
dc.date.available | 2020-05-28T16:43:28Z | |
dc.date.issued | 2019 | |
dc.identifier.citation | Boatman, T.G.; Geider, R.J. and
Oxborough, K. (2019) Improving
the Accuracy of Single Turnover
Active Fluorometry (STAF)
for the Estimation of Phytoplankton
Primary Productivity (PhytoPP).
Frontiers in Marine Science, 6:319, 16pp.
DOI: 10.3389/fmars.2019.00319 | en_US |
dc.identifier.uri | http://hdl.handle.net/11329/1346 | |
dc.identifier.uri | http://dx.doi.org/10.25607/OBP-852 | |
dc.description.abstract | Photosystem II (PSII) photochemistry is the ultimate source of reducing power
for phytoplankton primary productivity (PhytoPP). Single turnover active chlorophyll
fluorometry (STAF) provides a non-intrusive method that has the potential to measure
PhytoPP on much wider spatiotemporal scales than is possible with more direct
methods such as 14C fixation or O2 evolved through water oxidation. Application of
a STAF-derived absorption coefficient for PSII light-harvesting (aLHII) provides a method
for estimating PSII photochemical flux on a unit volume basis (JVPII). Within this study,
we assess potential errors in the calculation of JVPII arising from sources other than
photochemically active PSII complexes (baseline fluorescence) and the package effect.
Although our data show that such errors can be significant, we identify fluorescencebased
correction procedures that can be used to minimize their impact. For baseline
fluorescence, the correction incorporates an assumed consensus PSII photochemical
efficiency for dark-adapted material. The error generated by the package effect can be
minimized through the ratio of variable fluorescence measured within narrow wavebands
centered at 730 nm, where the re-absorption of PSII fluorescence emission is minimal,
and at 680 nm, where re-absorption of PSII fluorescence emission is maximal. We
conclude that, with incorporation of these corrective steps, STAF can provide a reliable
estimate of JVPII and, if used in conjunction with simultaneous satellite measurements
of ocean color, could take us significantly closer to achieving the objective of obtaining
reliable autonomous estimates of PhytoPP. | en_US |
dc.language.iso | en | en_US |
dc.rights | Attribution 4.0 International | * |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | * |
dc.subject.other | Photosynthesis | en_US |
dc.subject.other | Primary productivity | en_US |
dc.subject.other | Photosystem II | en_US |
dc.subject.other | Chlorophyll fluorescence | en_US |
dc.subject.other | Package effect | en_US |
dc.subject.other | Ocean colour | en_US |
dc.title | Improving the Accuracy of Single Turnover Active Fluorometry (STAF) for the Estimation of Phytoplankton Primary Productivity (PhytoPP). | en_US |
dc.type | Journal Contribution | en_US |
dc.description.refereed | Refereed | en_US |
dc.format.pagerange | 16pp. | en_US |
dc.identifier.doi | 10.3389/fmars.2019.00319 | |
dc.subject.parameterDiscipline | Parameter Discipline::Biological oceanography::Phytoplankton | en_US |
dc.bibliographicCitation.title | Frontiers in Marine Science | en_US |
dc.bibliographicCitation.volume | 6 | en_US |
dc.bibliographicCitation.issue | Article 319 | en_US |
dc.description.sdg | 14.A | en_US |
dc.description.eov | Phytoplankton biomass and diversity | en_US |
dc.description.maturitylevel | TRL 8 Actual system completed and "mission qualified" through test and demonstration in an operational environment (ground or space) | en_US |
dc.description.bptype | Manual (incl. handbook, guide, cookbook etc) | en_US |
dc.description.frontiers | 2019-06-14 | |
obps.contact.contactname | Kevin Oxborough | |
obps.contact.contactemail | koxborough@chelsea.co.uk | |
obps.resourceurl.publisher | https://www.frontiersin.org/articles/10.3389/fmars.2019.00319/full | en_US |