Improving the Accuracy of Single Turnover Active Fluorometry (STAF) for the Estimation of Phytoplankton Primary Productivity (PhytoPP).
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Boatman, Tobias G.
Geider, Richard J.
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Photosystem II (PSII) photochemistry is the ultimate source of reducing power for phytoplankton primary productivity (PhytoPP). Single turnover active chlorophyll fluorometry (STAF) provides a non-intrusive method that has the potential to measure PhytoPP on much wider spatiotemporal scales than is possible with more direct methods such as 14C fixation or O2 evolved through water oxidation. Application of a STAF-derived absorption coefficient for PSII light-harvesting (aLHII) provides a method for estimating PSII photochemical flux on a unit volume basis (JVPII). Within this study, we assess potential errors in the calculation of JVPII arising from sources other than photochemically active PSII complexes (baseline fluorescence) and the package effect. Although our data show that such errors can be significant, we identify fluorescencebased correction procedures that can be used to minimize their impact. For baseline fluorescence, the correction incorporates an assumed consen.....
JournalFrontiers in Marine Science
Sustainable Development Goals (SDG)14.A
Essential Ocean Variables (EOV)Phytoplankton biomass and diversity
Maturity LevelTRL 8 Actual system completed and "mission qualified" through test and demonstration in an operational environment (ground or space)
Best Practice TypeManual (incl. handbook, guide, cookbook etc)
CitationBoatman, T.G.; Geider, R.J. and Oxborough, K. (2019) Improving the Accuracy of Single Turnover Active Fluorometry (STAF) for the Estimation of Phytoplankton Primary Productivity (PhytoPP). Frontiers in Marine Science, 6:319, 16pp. DOI: 10.3389/fmars.2019.00319
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