Methodological Studies on Estimates of Abundance and Diversity of Heterotrophic Flagellates from the Deep-Sea Floor.

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Date
2016Author
Schoenle, Alexandra
Jeuck, Alexandra
Nitsche, Frank
Venter, Paul
Prausse, Dennis
Arndt, Hartmut
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Show full item recordAbstract
Extreme environmental conditions in the deep sea hamper access to protist communities.
In combination with the potentially highly diverse species composition, it demands a wide range
of methods to be applied at the same time to guarantee a high resolution of quantitative and
qualitative studies of deep-sea heterotrophic flagellates (HF). Within this study, we present a
possible combination of several culture-independent and culture-dependent methods available for
investigating benthic deep-sea HF communities. Besides live-counting and fixation of HF, we refer to
cultivation methods and molecular surveys using next generation sequencing. Laboratory ecological
experiments under deep-sea conditions (high pressure, low temperature) could allow the approval of
the potential deep-sea origin of sampled HF. The combination of different methods offers a unique
possibility to receive detailed information on nanofaunal life in the deep sea. Specific fixation
techniques to preserve sampl.....
Resource URL
https://www.mdpi.com/2077-1312/4/1/22Journal
Journal of Marine Science and EngineeringVolume
4Issue
Article 22Page Range
11pp.Document Language
enSustainable Development Goals (SDG)
14.AEssential Ocean Variables (EOV)
Invertebrate abundance and distributionMaturity Level
TRL 7 System prototyping demonstration in an operational environment (ground or space)Best Practice Type
Manual (incl. handbook, guide, cookbook etc)Standard Operating Procedure
DOI Original
10.3390/jmse4010022Citation
Schoenle, A.; Jeuck, A.; Nitsche, F.; Venter, P.; Prausse, D. and Arndt, H. (2016) Methodological Studies on Estimates of Abundance and Diversity of Heterotrophic Flagellates from the Deep-Sea Floor. Journal of Marine Science and Engineering, 4: 22, 11pp. DOI: 10.3390/jmse4010022Collections
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