Methodological Studies on Estimates of Abundance and Diversity of Heterotrophic Flagellates from the Deep-Sea Floor.
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Extreme environmental conditions in the deep sea hamper access to protist communities. In combination with the potentially highly diverse species composition, it demands a wide range of methods to be applied at the same time to guarantee a high resolution of quantitative and qualitative studies of deep-sea heterotrophic flagellates (HF). Within this study, we present a possible combination of several culture-independent and culture-dependent methods available for investigating benthic deep-sea HF communities. Besides live-counting and fixation of HF, we refer to cultivation methods and molecular surveys using next generation sequencing. Laboratory ecological experiments under deep-sea conditions (high pressure, low temperature) could allow the approval of the potential deep-sea origin of sampled HF. The combination of different methods offers a unique possibility to receive detailed information on nanofaunal life in the deep sea. Specific fixation techniques to preserve sampl.....
JournalJournal of Marine Science and Engineering
Sustainable Development Goals (SDG)14.A
Essential Ocean Variables (EOV)Invertebrate abundance and distribution
Maturity LevelTRL 7 System prototyping demonstration in an operational environment (ground or space)
Best Practice TypeManual (incl. handbook, guide, cookbook etc)
Standard Operating Procedure
CitationSchoenle, A.; Jeuck, A.; Nitsche, F.; Venter, P.; Prausse, D. and Arndt, H. (2016) Methodological Studies on Estimates of Abundance and Diversity of Heterotrophic Flagellates from the Deep-Sea Floor. Journal of Marine Science and Engineering, 4: 22, 11pp. DOI: 10.3390/jmse4010022
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