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dc.contributor.authorSchoenle, Alexandra
dc.contributor.authorJeuck, Alexandra
dc.contributor.authorNitsche, Frank
dc.contributor.authorVenter, Paul
dc.contributor.authorPrausse, Dennis
dc.contributor.authorArndt, Hartmut
dc.date.accessioned2020-05-11T20:12:05Z
dc.date.available2020-05-11T20:12:05Z
dc.date.issued2016
dc.identifier.citationSchoenle, A.; Jeuck, A.; Nitsche, F.; Venter, P.; Prausse, D. and Arndt, H. (2016) Methodological Studies on Estimates of Abundance and Diversity of Heterotrophic Flagellates from the Deep-Sea Floor. Journal of Marine Science and Engineering, 4: 22, 11pp. DOI: 10.3390/jmse4010022en_US
dc.identifier.urihttp://hdl.handle.net/11329/1327
dc.identifier.urihttp://dx.doi.org/10.25607/OBP-834
dc.description.abstractExtreme environmental conditions in the deep sea hamper access to protist communities. In combination with the potentially highly diverse species composition, it demands a wide range of methods to be applied at the same time to guarantee a high resolution of quantitative and qualitative studies of deep-sea heterotrophic flagellates (HF). Within this study, we present a possible combination of several culture-independent and culture-dependent methods available for investigating benthic deep-sea HF communities. Besides live-counting and fixation of HF, we refer to cultivation methods and molecular surveys using next generation sequencing. Laboratory ecological experiments under deep-sea conditions (high pressure, low temperature) could allow the approval of the potential deep-sea origin of sampled HF. The combination of different methods offers a unique possibility to receive detailed information on nanofaunal life in the deep sea. Specific fixation techniques to preserve samples directly at the sampling depth must be applied in further studies to reflect the real biodiversity of the largest habitat on earth.en_US
dc.language.isoenen_US
dc.rightsAttribution 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subject.otherNanofaunaen_US
dc.subject.otherProtisten_US
dc.subject.otherBenthosen_US
dc.subject.otherBenthic biodiversityen_US
dc.subject.otherLive countingen_US
dc.subject.otherLiquid aliquoten_US
dc.subject.otherFixationen_US
dc.titleMethodological Studies on Estimates of Abundance and Diversity of Heterotrophic Flagellates from the Deep-Sea Floor.en_US
dc.typeJournal Contributionen_US
dc.description.refereedRefereeden_US
dc.format.pagerange11pp.en_US
dc.identifier.doi10.3390/jmse4010022
dc.subject.parameterDisciplineParameter Discipline::Biological oceanography::Biota abundance, biomass and diversityen_US
dc.bibliographicCitation.titleJournal of Marine Science and Engineeringen_US
dc.bibliographicCitation.volume4en_US
dc.bibliographicCitation.issueArticle 22en_US
dc.description.sdg14.Aen_US
dc.description.eovInvertebrate abundance and distributionen_US
dc.description.maturitylevelTRL 7 System prototyping demonstration in an operational environment (ground or space)en_US
dc.description.bptypeManual (incl. handbook, guide, cookbook etc)en_US
dc.description.bptypeStandard Operating Procedureen_US
obps.contact.contactemailhartmut.arndt@uni-koeln.de;
obps.resourceurl.publisherhttps://www.mdpi.com/2077-1312/4/1/22en_US


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Attribution 4.0 International
Except where otherwise noted, this item's license is described as Attribution 4.0 International