Transcriptome sequencing of 19 diverse species of choanoflagellates.
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The origin of animals, which occurred over 600 million years ago, left no evidence in the fossil record. To trace the earliest events in animal prehistory, we compare extant animals to their closest living relatives, the choanoflagellates, in order to reconstruct the gene content of their last common ancestor and how it evolved on the stem lineage leading to animals. In this project, we increase the accuracy of ancestral animal and choanoflagellate gene content reconstructions by sequencing the transcriptomes of 19 species of choanoflagellates selected for their phylogenetic diversity. This protocol accompanies the following publication: Richter, Daniel J and Fozouni, Parinaz and Eisen, Michael and King, Nicole. Gene family innovation, conservation and loss on the animal stem lineage. 2018;7:e34226 https://doi.org/10.7554/eLife.34226.....
PublisherStation Biologique de Roscoff
Maturity LevelTRL 9 Actual system "mission proven" through successful mission operations (ground or space)
Best Practice TypeBest Practice
Standard Operating Procedure
CitationRichter, D.; Fozouni, P.; Eisen, M. and King, N. (2017) Transcriptome sequencing of 19 diverse species of choanoflagellates. Roscoff, France, Station Biologique de Roscoff, 1pp. DOI: dx.doi.org/10.17504/protocols.io.kwscxee
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