Simple 3D imaging of biogenic silica structures by fluorescence microscopy. Version 2.
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This protocol describes how to prepare, stain and mount biogenic silica particles (opaline from diatoms, Radiolaria, sponges spicules, phytolith...) for 3D imaging with fluorescence microscopy. The protocol is fast, convenient and affordable. Hence it could be a relevant alternative to X-ray computed tomography or photogrammetry......
PublisherStation Biologique de Roscoff
Maturity LevelTRL 9 Actual system "mission proven" through successful mission operations (ground or space)
Best Practice TypeBest Practice
Standard Operating Procedure
CitationColin, S. (2018) Simple 3D imaging of biogenic silica structures by fluorescence microscopy. Version 2. Roscoff, France, Station Biologique de Roscoff, 2pp. DOI: dx.doi.org/10.17504/protocols.io.vuze6x6
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