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dc.contributor.authorMoßhammer, Maria
dc.contributor.authorStrobl, Martin
dc.contributor.authorKühl, Michael
dc.contributor.authorKlimant, Ingo
dc.contributor.authorBorisov, Sergey M.
dc.contributor.authorKoren, Klaus
dc.identifier.citationMoßhammer, M.;Strobl, M.; Kühl, M.; Klimant, I.; Borisov, S.M.and Koren, K. (2016) Design and Application of an Optical Sensor for Simultaneous Imaging of pH and Dissolved O2 with Low Cross-Talk, ACS Sensors, 1, pp. 681-687. DOI: 10.1021/acssensors.6b00071en_US
dc.description.abstractVisualization and quantification of analytes such as O2 or pH is essential in biological research. Here we present the design and application of a new optical dual-analyte sensor for imaging, optimized to have low cross-sensitivity between the two analytes O2 and pH. The used indicator and reference dyes were selected to match the different channels of a commercial 2CCD (RGB + NIR) camera. A red-light emitting O2-sensitive europium complex (Eu(HPhN)3dpp) with a dynamic range of 0–20% O2 in the finished sensor was combined with a near-infrared emitting pH indicator (OHButoxy-aza-BODIPY) with a dynamic range of pH 7.2–8.8. To enable ratiometric readout, an inert reference coumarin dye (Bu3Coum) was co-immobilized with the optical indicators. In order to maximize the sensor signal, inert diamond powder was added to one sensor layer as a simple way to increase scattering of light within the sensor. Furthermore, the addition of an optical isolation layer enabled measurements in highly fluorescent samples, such as algal biofilms. The sensor was tested in a marine photosynthetic microbial mat.en_US
dc.rightsAttribution-NonCommercial 4.0*
dc.subject.otherChemical imagingen_US
dc.subject.otherDual-analyte sensoren_US
dc.subject.otherMicrobial maten_US
dc.subject.otherSensor applicationen_US
dc.titleDesign and Application of an Optical Sensor for Simultaneous Imaging of pH and Dissolved O2 with Low Cross-Talk.en_US
dc.typeJournal Contributionen_US
dc.subject.parameterDisciplineParameter Discipline::Chemical oceanographyen_US
dc.bibliographicCitation.titleACS Sensorsen_US
dc.description.bptypeStandard Operating Procedureen_US

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Attribution-NonCommercial 4.0
Except where otherwise noted, this item's license is described as Attribution-NonCommercial 4.0