Development of a Continuous Phytoplankton Culture System for Ocean Acidification Experiments.

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Date
2014Author
Wynn-Edwards, Cathryn
King, Rob
Kawaguchi, So
Davidson, Andrew
Wright, Simon
Nichols, Peter D.
Virtue, Patti
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Show full item recordAbstract
Around one third of all anthropogenic CO2 emissions have been absorbed by the
oceans, causing changes in seawater pH and carbonate chemistry. These changes have the
potential to affect phytoplankton, which are critically important for marine food webs and
the global carbon cycle. However, our current knowledge of how phytoplankton will
respond to these changes is limited to a few laboratory and mesocosm experiments.
Long-term experiments are needed to determine the vulnerability of phytoplankton to
enhanced pCO2. Maintaining phytoplankton cultures in exponential growth for extended
periods of time is logistically difficult and labour intensive. Here we describe a continuous
culture system that greatly reduces the time required to maintain phytoplankton cultures,
and minimises variation in experimental pCO2 treatments over time. This system is simple,
relatively cheap, flexible, and allows long-term experiments to be performed to further our
understanding of chronic responses.....
Resource URL
https://www.mdpi.com/2073-4441/6/6/1860/pdfJournal
WaterVolume
6Page Range
pp.860-1872Document Language
enSustainable Development Goals (SDG)
14.214.3
Essential Ocean Variables (EOV)
Phytoplankton biomass and diversityBest Practice Type
Best PracticeManual
DOI Original
10.3390/w6061860Citation
Wynn-Edwards, C.; King, R.; Kawaguchi, S.; Davidson, A.; Wright, S.; Nichols, P.D. and Virtue, P. (2014) Development of a Continuous Phytoplankton Culture System for Ocean Acidification Experiments. Water, 6, pp.1860-1872. DOI:10.3390/w6061860Collections
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