Repository of community practices in Ocean Research, Applications and Data/Information Management
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Earth Microbiome Project (EMP) high throughput (HTP) DNA extraction protocol. Version 07112018.
(University of California, San Diego, San Diego, CA, 2018)
This protocol accompanies the following publication: Marotz, Clarisse, et al. "DNA extraction for streamlined metagenomics of diverse environmental samples." BioTechniques 62.6 (2017):290-293
Quanti-iT™ Pico Green dsDNA Assay (Invitrogen P7589).
(Matthew Sullivan Lab, University of Arizona/Ohio State University, Tucson, AZ, 2019)
This protocol accompanies the following publication: Ul-Hasan S, Bowers RM, Figueroa-Montiel A, Licea-Navarro AF, Beman JM, Woyke T, et al. (2019) Community ecology across bacteria, archaea and microbial eukaryotes in the ...
Scanning Electron Microscopy imaging for Opaline Silica Single Cell Skeletons (Polycystines Radiolaria). Version 3.
(Station Biologique de Roscoff, Roscoff, France, 2018)
Protocol adapted from:
Biard, T., Pillet, L., Decelle, J., Poirier, C., Suzuki, N. and Not, F. (2015) Towards an Integrative Morpho-molecular Classification of the Collodaria (Polycystinea, Radiolaria). Protist 166, ...
Transcriptome sequencing of 19 diverse species of choanoflagellates.
(Station Biologique de Roscoff, Roscoff, France, 2017)
The origin of animals, which occurred over 600 million years ago, left no evidence in the fossil record. To trace the earliest events in animal
prehistory, we compare extant animals to their closest living relatives, the ...
Confocal Microscopy imaging for Opaline Silica Single Cell Skeletons (Polycystines Radiolaria).
(Station Biologique de Roscoff, Roscoff, France, 2018)
Single cell rDNA amplification of Nassellaria (Radiolaria).
(Station Biologique de Roscoff, Roscoff, France, 2018)
EMP Sample Submission Guide.
(NOAA Fisheries Southwest Fisheries Research Center, La Jolla,CA, 2018)
This protocol was designed for collaborators with the Earth Microbiome Project to contribute samples in a standardized fashion. Raw, frozen samples are submitted in individually labeled tubes, 10 aliquots (identical ...
Toward a Global Public Repository of Community Protocols to Encourage Best Practices in Biomolecular Ocean Observing and Research
(2021)
Biomolecular ocean observing and research is a rapidly evolving field that uses omics approaches to describe biodiversity at its foundational level, giving insight into the structure and function of marine ecosystems over ...
Expanding "Tara" Oceans Protocols for Underway, Ecosystemic Sampling of the Ocean-Atmosphere Interface During Tara Pacific Expedition (2016–2018).
(2019)
Interactions between the ocean and the atmosphere occur at the air-sea interface
through the transfer of momentum, heat, gases and particulate matter, and through
the impact of the upper-ocean biology on the composition ...