HPLC Chlorophyll Processing for the ACT Performance Verification of In Situ Fluorometers.
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Alliance for Coastal Technologies
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The HPLC method used for chlorophyll a analysis followed that of Zapata et al (2000; MEPS 195:29-45) and was conducted by the laboratory of Dr. Welschmeyer at Moss Landing Marine Laboratories (MLML, the West Coast ACT Partner Institution). All pigment samples from Partner sites were collected quantitatively on Whatman 2.5 cm GF/F filters, frozen in liquid N2 and shipped by overnight courier in liquid N2 dry shippers to MLML. Samples were removed from liquid N2, extracted overnight in quantitative volumes (1.8 – 2.0 mL) of N2-purged 90% acetone (-20 oC) and ground with a motorized Teflon tissue grinder the following morning (the soak-and-grind method). Samples were kept on ice until loaded on HPLC autosamplers (three identical HPLC systems were used during this project). Replicate test samples supplied by Partner sites showed that extraction by the soak-and-grind method yielded as much as 25% more chl a than by routine acetone soaking alone. Prior to grinding, all samples and.....
PublisherAlliance for Coastal Technologies (ACT)
Essential Ocean Variables (EOV)Ocean colour
Best Practice TypeBest Practice
Standard Operating Procedure
CitationAlliance for Coastal Technology (2004) HPLC Chlorophyll Processing for the ACT Performance Verification of In Situ Fluorometers. Solomons, MD, Alliance for Coastal Technologies, 1pp. DOI: http://dx.doi.org/10.25607/OBP-360
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