Chlorophyll a: Determination by spectroscopic methods.
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Chlorophyll a is the principal pigment in plants. As a biomass indicator of aquatic microalgae which support food webs in the sea, it is probably the most frequently measured biochemical parameter in oceanography. This document describes a procedure for the routine determination of chlorophyll a in sea water for use by ICES Member Countries. It has been developed from a review of current methodology for measuring chlorophyll a undertaken by the Working Group on Phytoplankton Ecology (WGPE) and the Marine Chemistry Working Group (MCWG). This work was led by A. Aminot (MCWG) and F. Rey (WGPE) and both groups based their discussions around a recently published work on this topic (Jeffrey, Mantoura, and Wright, 1997). The present document represents the consensus between the two groups. This document draws attention to critical points of chlorophyll a determination and reviews recommendations concerning the use of this pigment as a biomass marker. In addition, it proposes a standard .....
PublisherInternational Council for the Exploration of the Sea (ICES)
Series;NrICES Techniques in Marine Environmental Sciences; 30
Essential Ocean Variables (EOV)Ocean colour
Phytoplankton biomass and diversity
Best Practice TypeStandard Operating Procedure
CitationAminot, A., and Rey, F. (2001) Chlorophyll a: Determination by spectroscopic methods. ICES Techniqnes in Marine Environmental Sciences, No. 30, 17pp. DOI: http://dx.doi.org/10.25607/OBP-278