Biological effects of contaminants: Quantification of metallothionein (MT) in fish liver tissue.
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This document describes methods to analyse the protein metallothionein in fish tissues. Metallothionein is induced by and binds essential (Cu, Zn) and non-essential (Cd, Hg) metals and is used in monitoring programmes as a marker for environmental metal exposure. The main focus is on the use and development of immunochemical procedures (ELISA). In addition, two alternative methods, electrochemical and spectrophotometric, are described......
PublisherInternational Council for the Exploration of the Sea (ICES)
Series;NrICES Techniques in Marine Environmental Sciences; 26
Sustainable Development Goals (SDG)14.1
Best Practice TypeStandard Operating Procedure
CitationHylland, K. (1999) Biological effects of contaminants: Quantification of metallothionein (MT) in fish liver tissue. ICES Techniques in Marine Environmental Sciences, No. 26, 18pp. DOI: http://dx.doi.org/10.25607/OBP-273