Biological effects of contaminants: Microplate method for measurement of ethoxyresorufin-O-deethylase (EROD) in fish.
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Payne, J. F.
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Interest in the use of mixed function oxidase (MFa) as a monitoring tool for measuring the effects of pollntants derives from basic research carried out over the past twenty years (see review in Payne et al., 1987). The MFa system catalyses the degradation of both endogenous and exogenous lipophilic substrates to polar water-soluble products which are more easily excreted. It is present at relatively low activity in wild fish and its activity increases dramatically, apparently to enhance the degradation and clearance of offending compounds. This suggests that the activity of the MFa system in naturally contaminated organisms might be a measure of the degree of chemical contamination. There have been a number of field studies in which elevated MFa activity in fish was found to be associated with contamination by hydrocarbons (payne et al., 1987). The MFa .....
PublisherInternational Council for the Exploration of the Sea (ICES)
Series;NrICES Techniques in Marine Environmental Sciences; 13
Sustainable Development Goals (SDG)14.1
Best Practice TypeStandard Operating Procedure
CitationGalgani, F. and Payne, J.F. (1991) Biological effects of contaminants: Microplate method for measurement of ethoxyresorufin-O-deethylase (EROD) in fish. ICES Techniques in Marine Environmental Sciences, No. 13, 11pp. DOI: http://dx.doi.org/10.25607/OBP-248