Lysosomal membrane stability in mussels.
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In 2012, the ICES Study Group on Integrated Monitoring of Chemicals and their Effects provided a framework for integrated monitoring to the OSLO-Paris Commission. UNEP/MAP and HELCOM expert groups have also developed guidelines on integrated monitoring of chemicals and their effects for the Mediterranean and Baltic Sea. This document provides the technical information for one of the biological effects measurements, the lysosomal membrane stability (LMS), which is a part of the above mentioned integrated monitoring approaches. Lysosomes are cytoplasmic, single membrane organelles whose condition is sensitive to stress whether it be due to environmental conditions or exposure to a wide array of contaminants. Two different methodologies have been developed to assess LMS in mussels: an enzyme cytochem- ical method using cryostatic sections of digestive gland tissue, and an in vivo cyto- chemical method (using haemolymph cells). In this document, different aspects of the operational procedu.....
PublisherInternational Council for the Exploration of the Sea (ICES)
Series;NrICES Techniques in Marine Environmental Sciences; 56
Sustainable Development Goals (SDG)14.1
Best Practice TypeStandard Operating Procedure
CitationMartínez-Gómez, C.; Bignell, J. and Lowe, D. (2015) Lysosomal membrane stability in mussels. ICES Techniques in Marine Environmental Sciences, No. 56, 41pp. DOI: http://dx.doi.org/10.25607/OBP-240