Determination of alkyl phenol metabolites in fish bile using solid-phase analytical derivatization (SPAD) and gas chromatography-mass spectrometry in electron ionization mode (GC-EI-MS).

Abstract

This document provides advice on the analysis of alkylphenol (AP) metabolites in fish bile. APs are released to aquatic environments from many different sources related to human activities, such as offshore oil production. The method for determination of APs includes enzymatic deconjugation of fish bile followed by solid‐phase analytical derivatization (SPAD) with bis(trimethylsilyl)‐ trifluoroacetamide (BSTFA). The derivatized APs are separated, then quantified using gas chromatography–mass spectrometry in the electron ionization mode (GC– EI–MS). Quality control measures should be implemented to ensure good performance of the method. This GC–EI–MS method allows for a selective and sensitive analytical detection of APs in fish bile.