Biological effects of contaminants: Quantification of δ-aminolevulinic acid dehydratase (ALA-D) activity in fish blood.
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This document describes a colorimetric method to quantify the enzyme δ-aminolevulinic acid dehydratase (ALA-D) in fish blood. ALA-D is an enzyme in the heme synthesis pathway. The activity of the enzyme is inhibited by lead (Pb) and it has, therefore, been used as a biomarker for lead exposure and effects in mammals, birds, and fish. This paper describes optimal conditions for the kinetic determination of ALA-D activity in fish blood, standardized to protein content......
PublisherInternational Council for the Exploration of the Sea (ICES)
Series;NrICES Techniques in Marine Environmental Sciences;34
Sustainable Development Goals (SDG)14.1
Essential Ocean Variables (EOV)Fish abundance and distribution
Best Practice TypeStandard Operating Procedure
CitationHylland, K. (2004) Biological effects of contaminants: Quantification of δ-aminolevulinic acid dehydratase (ALA-D) activity in fish blood. ICES Techniques in Marine Environmental Sciences, No. 34. 9pp. DOI: http://dx.doi.org/10.25607/OBP-229