Biological effects of contaminants: measurement of lysosomal membrane stability.
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Lysosomes are ubiquitous cellular organelles that provide a waste disposal and macromolecular recycling system (autophagy) and also a membrane-bound compartment for intracellular digestion of food ingested by the cells. They accumulate many toxic metals and organic chemical contaminants, providing an evolutionarily primitive detoxication capacity, which if overloaded results in lysosomal damage leading to cell injury, tissue dysfunction, and reduction in animal “health status”. Major reactions of lysosomes to pollutants include loss of membrane integrity, enlargement associated with autophagy, and accumulation of lipid and lipofuscin (agepigment). These types of responses have been widely used to test for the effects of toxic contaminants in both experimental investigations and environmental impact assessments. Several methods are available to measure lysosomal functional status: these include measurement of lysosomal membrane stability in both frozen tissue sections and live.....
PublisherInternational Council for the Exploration of the Sea (ICES)
Series;NrICES Techniques in Marine Environmental Sciences;36
Sustainable Development Goals (SDG)14.A
Essential Ocean Variables (EOV)Microbe biomass and diversity
Best Practice TypeStandard Operating Procedure
CitationMoore, M.N.; Lowe,D. and Köhler, A. (2004) Biological effects of contaminants: measurement of lysosomal membrane stability. ICES Techniques in Marine Environmental Sciences, No. 36. 31pp. DOI: http://dx.doi.org/10.25607/OBP-227