Review of analytical methods for determining metabolites of polycyclic aromatic compounds (PACs) in fish bile.
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In fish, metabolites of polycyclic aromatic compounds (PACs) in gall bladder bile can be used as biomarkers for recent environmental exposure to PACs. These metabolites in the bile result from hepatic biotransformation processes whereby the lipophilic parent PACs are transformed in two steps (hydroxylation and subsequent conjugation) to more soluble forms and then passed to the gall bladder for elimination from the organism. As a biomarker of exposure, the determination of PAC metabolites in bile has several advantages over other assessment techniques. Several bile PACs are strong fluorophores and can thus be measured semi-quantitatively and very easily by means of straightforward fluorescence detection techniques. For example, fixed fluorescence detection and synchronous fluorescence scanning can be used for this purpose. These techniques are excellent for rapid screening of overall PAC exposure levels, but less suitable for the determination of individual compounds. The next level of.....
PublisherInternational Council for the Exploration of the Sea (ICES)
Series;NrICES Techniques in Marine Environmental Sciences;39
Sustainable Development Goals (SDG)14.A
Best Practice TypeStandard Operating Procedure
CitationAriese, F.; Beyer, J.; Jonsson, G.; Porte, C. and Krahn, M.M. (2005) Review of analytical methods for determining metabolites of polycyclic aromatic compounds (PACs) in fish bile. ICES Techniques in Marine Environmental Sciences, No. 39, 41pp. DOI: http://dx.doi.org/10.25607/OBP-225