Critical considerations for the application of environmental DNA methods to detect aquatic species.

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Date
2016Author
Goldberg, Caren S.
Turner, Cameron R.
Deiner, Kristy
Klymus, Katy E.
Thomsen, Philip Francis
Murphy, Melanie A.
Spear, Stephen F.
McKee, Anna
Oyler‐McCance, Sara J.
Cornman, Robert Scott
Laramie, Matthew B.
Mahon, Andrew R.
Lance, Richard F.
Pilliod, David S.
Strickler, Katherine M.
Waits, Lisette P.
Fremier, Alexander K.
Takahara, Teruhiko
Herder, Jelger E.
Taberlet, Pierre
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1. Species detection using environmental DNA (eDNA) has tremendous potential for contributing to the understanding of the ecology and conservation of aquatic species. Detecting species using eDNA methods, rather than
directly sampling the organisms, can reduce impacts on sensitive species and increase the power of field surveys
for rare and elusive species. The sensitivity of eDNA methods, however, requires a heightened awareness and
attention to quality assurance and quality control protocols. Additionally, the interpretation of eDNA data
demands careful consideration of multiple factors. As eDNA methods have grown in application, diverse
approaches have been implemented to address these issues. With interest in eDNA continuing to expand, supportive guidelines for undertaking eDNA studies are greatly needed.
2. Environmental DNA researchers from around the world have collaborated to produce this set of guidelines
and considerations for implementing eDNA methods to detect aquati.....
Journal
Methods in Ecology and EvolutionVolume
7Page Range
pp.299-1307Document Language
enBest Practice Type
Best PracticeGuide
DOI Original
10.1111/2041-210X.12595Citation
Goldberg, C. S.; Turner, C. R.; Deiner, K. ; Klymus, K. E.; Thomsen, P. F.; Murphy, M. A.; Spear, S. F.; McKee, A.; Oyler‐McCance, S. J.; Cornman, R. S.; Laramie, M. B.,; Mahon, A. R.; Lance, R. F.; Pilliod, D. S.; Strickler, K. M.; Waits, L. P;, Fremier, A. K.; Takahara, T.; Herder, J. E. and Taberlet, P. (2016) Critical considerations for the application of environmental DNA methods to detect aquatic species. Methods in Ecology and Evolution, 7, pp.1299-1307. DOI: 10.1111/2041-210X.12595Collections
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