User's Manual: Flexible Fluidics for Genomics, Module Operation. Version 1.0.

Abstract

This document is the User’s Manual for the TechOceanS Flexible Fluidics Genomics Analysis System. The term ‘System’ refers to the collective use of Flexible Fluidics Modules and associated apparatus for the purpose of obtaining a purified DNA sample and analysing that DNA sample by nucleic acid amplification and fluorometric detection of a target sequence. It will describe the operational steps necessary to complete a genomics analysis, including DNA purification and DNA sequence amplification and detection on prototype desktop apparatus. This apparatus is intended for the validation of the flexible fluidics technology for the purpose of DNA purification and analysis, and as a ‘tool’ to assist the team in the development and optimisation of the technology. It does not constitute a fully functional or deployable system. The apparatus can be used with Flexible Fluidics modules for DNA purification and for DNA amplification for the measurement of a range of target species and covering many different applications; the relevant DNA amplification assays and chemistries have been designed, optimised and validated through the TechOceanS project. Information is given in the D3.1 and D8.1 reports.