Best practices for generating and analyzing 16S rRNA amplicon data to track coral microbiome dynamics.
![Thumbnail](/bitstream/handle/11329/2408/fmicb-13-1007877.pdf.jpg?sequence=6&isAllowed=y)
View/ Open
Average rating
votes
Date
2023Author
Silva, Denise P.
Epstein, Hannah E.
Vega Thurber, Rebecca L.
Metadata
Show full item recordAbstract
Over the past two decades, researchers have searched for methods to better
understand the relationship between coral hosts and their microbiomes. Data on
how coral-associated bacteria are involved in their host’s responses to stressors
that cause bleaching, disease, and other deleterious effects can elucidate how they
may mediate, ameliorate, and exacerbate interactions between the coral and the
surrounding environment. At the same time tracking coral bacteria dynamics can
reveal previously undiscovered mechanisms of coral resilience, acclimatization,
and evolutionary adaptation. Although modern techniques have reduced the cost
of conducting high-throughput sequencing of coral microbes, to explore the
composition, function, and dynamics of coral-associated bacteria, it is necessary
that the entire procedure, from collection to sequencing, and subsequent analysis
be carried out in an objective and effective way. Corals represent a difficult host
with which to work, and uniqu.....
Journal
Frontiers in MicrobiologyVolume
13:. doi:Issue
1007877Page Range
18pp.Document Language
enSustainable Development Goals (SDG)
14.aMaturity Level
MatureDOI Original
https://doi.org/10.3389/fmicb.2022.1007877Citation
Silva DP, Epstein HE and Vega Thurber RL (2023) Best practices for generating and analyzing 16S rRNA amplicon data to track coral microbiome dynamics. Frontiers in Microbiology, 13:1007877, 18pp. DOI: | https://doi.org/10.3389/fmicb.2022.1007877Collections
The following license files are associated with this item: