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dc.contributor.authorGerdes, Birte
dc.contributor.authorBrinkmeyer, Robin
dc.contributor.authorDieckmann, Gerhard
dc.contributor.authorHelmke, Elisabeth
dc.coverage.spatialArctic Oceanen_US
dc.identifier.citationGerdes, B., Brinkmeyer, R., Dieckmann, G. and Helmke, E. (2005) Influence of crude oil on changes of bacterial communities in Arctic sea-ice. FEMS Microbiology Ecology, 53:515893, pp.129–139. DOI:
dc.description.abstractThe danger of a petroleum hydrocarbon spillage in the polar, ice-covered regions is increasing due to oil exploration in Arctic offshore areas and a growing interest in using the Northern Sea Route (NSR) as an alternative transportation route for Arctic oil and gas. However, little is known about the potential impact of accidental oil spills on this environment. We investigated the impact of crude oil on microbial community composition in six different Arctic sea-ice samples incubated with crude oil at 1 degrees C in microcosms for one year. Alterations in the composition of bacterial communities were analyzed with the culture-independent molecular methods DGGE (denaturing gradient gel electrophoresis) and FISH (fluorescence in situ hybridization). DGGE, FISH and cultivation methods revealed a strong shift in community composition toward the gamma-proteobacteria in sea-ice and melt pool samples incubated with crude oil. Marinobacter spp., Shewanella spp. and Pseudomonas spp. were the predominant phylotypes in the oil-trealed microcosms. The ability of indigenous sea-ice bacteria to degrade hydrocarbons at low temperature (1 degrees C) was tested using four representative strains cultivated from sea-ice enriched with crude oil. [C-14]Hexadecane was degraded by the sea-ice isolates at 20-50% capacity of the mesophilic type strain Marinobacter hydrocarbonoclasticus, a known hydrocarbon degrader, incubated at 22 degrees C. (c) 2004 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.en_US
dc.rightsAttribution 4.0 International*
dc.subject.otherSea Iceen_US
dc.subject.otherOil pollutionen_US
dc.subject.otherOil degradationen_US
dc.subject.otherLow temperatureen_US
dc.subject.otherBacterial communities
dc.titleInfluence of crude oil on changes of bacterial communities in Arctic sea-ice.en_US
dc.typeJournal Contributionen_US
dc.subject.dmProcessesData analysisen_US
dc.bibliographicCitation.titleFEMS Microbiology Ecologyen_US
dc.description.methodologyTypeReports with methodological relevanceen_US Gerdes

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Attribution 4.0 International
Except where otherwise noted, this item's license is described as Attribution 4.0 International