A novel cost effective and high-throughput isolation and identification method for marine microalgae.
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Jahn, Martin T.
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Background: Marine microalgae are of major ecologic and emerging economic importance. Biotechnological screening schemes of microalgae for specific traits and laboratory experiments to advance our knowledge on algal biology and evolution strongly benefit from culture collections reflecting a maximum of the natural inter-and intraspecific diversity. However, standard procedures for strain isolation and identification, namely DNA extraction, purification, amplification, sequencing and taxonomic identification still include considerable constraints increasing the time required to establish new cultures. Results: In this study, we report a cost effective and high-throughput isolation and identification method for marine microalgae. The throughput was increased by applying strain isolation on plates and taxonomic identification by direct PCR (dPCR) of phylogenetic marker genes in combination with a novel sequencing electropherogram based screening method to assess the taxonomic diversity an.....
Sustainable Development Goals (SDG)14.a
Spatial CoverageArctic Ocean
CitationJahn, M. T., Schmidt, K. and Mock, T. (2014) A novel cost effective and high-throughput isolation and identification method for marine microalgae. Plant Methods, 10: 26, 10pp. DOI: https://doi.org/10.1186/1746-4811-10-26
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