Roadmap Towards Communitywide Intercalibration and Standardization of Ocean Nucleic Acids ‘Omics Measurements : Ocean Nucleic Acids ‘Omics Intercalibration and Standardization Workshop University of North Carolina, Chapel Hill, NC, USA January 8-11, 2020.
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Santoro, Alyson E.
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In January 2020, the US Ocean Carbon & Biogeochemistry (OCB) Project Office funded the Ocean Nucleic Acids 'omics Intercalibration and Standardization workshop held at the University of North Carolina in Chapel Hill. Thirty-two participants from across the US, along with guests from Canada and France, met to develop a framework for standardization and intercalibration (S&I) of ocean nucleic acid ‘omics (na’omics) approaches (i.e., amplicon sequencing, metagenomics and metatranscriptomics). During the three-day workshop, participants discussed numerous topics, including: a) sample biomass collection and nucleic acid preservation for downstream analysis, b) extraction protocols for nucleic acids, c) addition of standard reference material to nucleic acid isolation protocols, d) isolation methods unique to RNA, e) sequence library construction, and f ) integration of bioinformatic considerations. This report provides a summary of these and other topics covered during the worksho.....
PublisherOcean Carbon & Biogeochemistry Program
Woods Hole, MA
Sustainable Development Goals (SDG)14.a
CitationBerube, P., Gifford, S., Hurwitz, B., Jenkins, B., Marchetti, A. and Santoro, A.E. (2022) Roadmap Towards Communitywide Intercalibration and Standardization of Ocean Nucleic Acids ‘Omics Measurements: Ocean Nucleic Acids ‘Omics Intercalibration and Standardization Workshop, University of North Carolina, Chapel Hill, NC, USA, January 8-11, 2020. Woods Hole, MA, Ocean Carbon & Biogeochemistry Program, 49pp. DOI: 10.1575/1912/28054