DNA Metabarcoding Methods for the Study of Marine Benthic Meiofauna: A Review.
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Costa, Filipe Oliveira
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Meiofaunal animals, roughly between 0.045 and 1 mm in size, are ubiquitous and ecologically important inhabitants of benthic marine ecosystems. Their high species richness and rapid response to environmental change make them promising targets for ecological and biomonitoring studies. However, diversity patterns of benthic marine meiofauna remain poorly known due to challenges in species identification using classical morphological methods. DNA metabarcoding is a powerful tool to overcome this limitation. Here, we review DNA metabarcoding approaches used in studies on marine meiobenthos with the aim of facilitating researchers to make informed decisions for the implementation of DNA metabarcoding in meiofaunal biodiversity monitoring. We found that the applied methods vary greatly between researchers and studies, and concluded that further explicit comparisons of protocols are needed to apply DNA metabarcoding as a standard tool for assessing benthic meiofaunal community com.....
JournalFrontiers in Marine Science
Sustainable Development Goals (SDG)14.a
CitationGielings, R., Fais, M,. Fontaneto, D., Creer ,S., Costa, F.O., et al (2021) DNA Metabarcoding Methods for the Study of Marine Benthic Meiofauna: A Review. Frontiers in Marine Science, 8:730063, 13pp. DOI: 10.3389/fmars.2021.730063
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