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dc.contributor.authorKraft, Kaisa
dc.contributor.authorSeppälä, Jukka
dc.contributor.authorHällfors, Heidi
dc.contributor.authorSuikkanen, Sanna
dc.contributor.authorYlöstalo, Pasi
dc.contributor.authorAnglès, Sílvia
dc.contributor.authorKielosto, Sami
dc.contributor.authorKuosa, Harri
dc.contributor.authorLaakso, Lauri
dc.contributor.authorHonkanen, Martti
dc.contributor.authorLehtinen, Sirpa
dc.contributor.authorOja, Johanna
dc.contributor.authorTamminen, Timo
dc.coverage.spatialBaltic Seaen_US
dc.identifier.citationKraft, K, Seppälä, J, Hällfors, H, Suikkanen ,S, Ylöstalo, P., et al (2021) First Application of IFCB High-Frequency Imaging-in-Flow Cytometry to Investigate Bloom-Forming Filamentous Cyanobacteria in the Baltic Sea. Frontiers in Marine Science, 8:594144, 17pp. DOI: 10.3389/fmars.2021.594144en_US
dc.description.abstractCyanobacteria are an important part of phytoplankton communities, however, they are also known for forming massive blooms with potentially deleterious effects on recreational use, human and animal health, and ecosystem functioning. Emerging high frequency imaging flow cytometry applications, such as Imaging FlowCytobot (IFCB), are crucial in furthering our understanding of the factors driving bloom dynamics, since these applications provide community composition information at frequencies impossible to attain using conventional monitoring methods. However, the proof of applicability of automated imaging applications for studying dynamics of filamentous cyanobacteria is still scarce. In this study we present the first results of IFCB applied to a Baltic Sea cyanobacterial bloom community using a continuous flow-through setup. Our main aim was to demonstrate the pros and cons of the IFCB in identifying filamentous cyanobacterial taxa and in estimating their biomass. Selected environmental parameters (water temperature, wind speed and salinity) were included, in order to demonstrate the dynamics of the system the cyanobacteria occur in and the possibilities for analyzing high-frequency phytoplankton observations against changes in the environment. In order to compare the IFCB results with conventional monitoring methods, filamentous cyanobacteria were enumerated from water samples using light microscopical analysis. Two common bloom forming filamentous cyanobacteria in the Baltic Sea, Aphanizomenon flosaquae and Dolichospermum spp. dominated the bloom, followed by an increase in Oscillatoriales abundance. The IFCB results compared well with the results of the light microscopical analysis, especially in the case of Dolichospermum. Aphanizomenon biomass varied slightly between the methods and the Oscillatoriales results deviated the most. Bloom formation was initiated as water temperature increased to over 15C and terminated as the wind speed increased, dispersing the bloom. Community shifts were closely related to movements of the water mass. We demonstrate how using a high-frequency imaging flow cytometry application can help understand the development of cyanobacteria summer blooms.en_US
dc.description.sponsorshipJERICO-NEXT , JERICO-S3
dc.description.sponsorshipEuropean Union
dc.rightsAttribution 4.0 International*
dc.subject.otherImaging FlowCytoboten_US
dc.subject.otherHigh-frequency observationsen_US
dc.subject.otherBloom dynamicsen_US
dc.subject.otherImaging-in-flow cytometryen_US
dc.titleFirst Application of IFCB High-Frequency Imaging-in-Flow Cytometry to Investigate Bloom-Forming Filamentous Cyanobacteria.en_US
dc.typeJournal Contributionen_US
dc.subject.instrumentTypeFlow cytometersen_US
dc.bibliographicCitation.titleFrontiers in Marine Scienceen_US
dc.bibliographicCitation.issueArticle 594144en_US
dc.description.adoptionNovel (no adoption outside originators)en_US Kraft

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Attribution 4.0 International
Except where otherwise noted, this item's license is described as Attribution 4.0 International