dc.contributor.author | Kraft, Kaisa | |
dc.contributor.author | Seppälä, Jukka | |
dc.contributor.author | Hällfors, Heidi | |
dc.contributor.author | Suikkanen, Sanna | |
dc.contributor.author | Ylöstalo, Pasi | |
dc.contributor.author | Anglès, Sílvia | |
dc.contributor.author | Kielosto, Sami | |
dc.contributor.author | Kuosa, Harri | |
dc.contributor.author | Laakso, Lauri | |
dc.contributor.author | Honkanen, Martti | |
dc.contributor.author | Lehtinen, Sirpa | |
dc.contributor.author | Oja, Johanna | |
dc.contributor.author | Tamminen, Timo | |
dc.coverage.spatial | Baltic Sea | en_US |
dc.date.accessioned | 2021-11-26T20:13:38Z | |
dc.date.available | 2021-11-26T20:13:38Z | |
dc.date.issued | 2021 | |
dc.identifier.citation | Kraft, K, Seppälä, J, Hällfors, H,
Suikkanen ,S, Ylöstalo, P., et al (2021) First Application
of IFCB High-Frequency
Imaging-in-Flow Cytometry
to Investigate Bloom-Forming
Filamentous Cyanobacteria
in the Baltic Sea.
Frontiers in Marine Science, 8:594144, 17pp. DOI: 10.3389/fmars.2021.594144 | en_US |
dc.identifier.uri | https://repository.oceanbestpractices.org/handle/11329/1798 | |
dc.description.abstract | Cyanobacteria are an important part of phytoplankton communities, however, they
are also known for forming massive blooms with potentially deleterious effects on
recreational use, human and animal health, and ecosystem functioning. Emerging high frequency
imaging flow cytometry applications, such as Imaging FlowCytobot (IFCB),
are crucial in furthering our understanding of the factors driving bloom dynamics,
since these applications provide community composition information at frequencies
impossible to attain using conventional monitoring methods. However, the proof of
applicability of automated imaging applications for studying dynamics of filamentous
cyanobacteria is still scarce. In this study we present the first results of IFCB applied
to a Baltic Sea cyanobacterial bloom community using a continuous flow-through
setup. Our main aim was to demonstrate the pros and cons of the IFCB in identifying
filamentous cyanobacterial taxa and in estimating their biomass. Selected environmental
parameters (water temperature, wind speed and salinity) were included, in order
to demonstrate the dynamics of the system the cyanobacteria occur in and the
possibilities for analyzing high-frequency phytoplankton observations against changes
in the environment. In order to compare the IFCB results with conventional monitoring
methods, filamentous cyanobacteria were enumerated from water samples using light
microscopical analysis. Two common bloom forming filamentous cyanobacteria in the
Baltic Sea, Aphanizomenon flosaquae and Dolichospermum spp. dominated the bloom,
followed by an increase in Oscillatoriales abundance. The IFCB results compared
well with the results of the light microscopical analysis, especially in the case of
Dolichospermum. Aphanizomenon biomass varied slightly between the methods and
the Oscillatoriales results deviated the most. Bloom formation was initiated as water
temperature increased to over 15C and terminated as the wind speed increased,
dispersing the bloom. Community shifts were closely related to movements of the water
mass. We demonstrate how using a high-frequency imaging flow cytometry application
can help understand the development of cyanobacteria summer blooms. | en_US |
dc.description.sponsorship | JERICO-NEXT , JERICO-S3 | |
dc.description.sponsorship | European Union | |
dc.language.iso | en | en_US |
dc.rights | Attribution 4.0 International | * |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | * |
dc.subject.other | IFCB | en_US |
dc.subject.other | Imaging FlowCytobot | en_US |
dc.subject.other | High-frequency observations | en_US |
dc.subject.other | Cyanobacteria | en_US |
dc.subject.other | Cyanophyte | en_US |
dc.subject.other | Bloom dynamics | en_US |
dc.subject.other | Imaging-in-flow cytometry | en_US |
dc.title | First Application of IFCB High-Frequency Imaging-in-Flow Cytometry to Investigate Bloom-Forming Filamentous Cyanobacteria. | en_US |
dc.type | Journal Contribution | en_US |
dc.description.refereed | Refereed | en_US |
dc.identifier.doi | 10.3389/fmars.2021.594144 | |
dc.subject.parameterDiscipline | Phytoplankton | en_US |
dc.subject.instrumentType | Flow cytometers | en_US |
dc.bibliographicCitation.title | Frontiers in Marine Science | en_US |
dc.bibliographicCitation.volume | 8 | en_US |
dc.bibliographicCitation.issue | Article 594144 | en_US |
dc.description.sdg | 14.a | en_US |
dc.description.eov | N/A | en_US |
dc.description.adoption | Novel (no adoption outside originators) | en_US |
obps.contact.contactname | Kaisa Kraft | |
obps.contact.contactemail | kaisa.kraft@syke.fi | |