A Simple and Inexpensive Method for Manipulating Dissolved Oxygen in the Lab.

Abstract

Changes in dissolved oxygen concentration can cause dramatic shifts in chemical, biological, and ecological processes in aquatic systems. In shallow coastal areas, this can happen on short timescales, with oxygen increasing during the day due to photosynthesis and declining at night due to respiration. We present a system controlled by an Arduino microprocessor that leverages the oxygen-consuming capacity of sediments to manipulate dissolved oxygen in an aquarium tank to planned concentrations. With minor adjustments to the Arduino code, the system can produce a variety of dissolved oxygen patterns, including a diel cycle. Designed to be user-friendly and scalable if needed, the system uses easily acquired, low-cost electronic and aquarium components. Its simplicity and accessibility permit deeper exploration of the effects of dissolved oxygen variability in aquatic systems, and the use of Arduino code and basic electronics makes it a potential tool for teaching experimental design and instrument fabrication.