Method for Reproducible Shipboard Segmented Flow Analysis Ammonium Measurement using an In-House Reference Material for Quality Control.
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Ammonium is a fundamental nutrient for phytoplankton growth in seawater and is a key component of the microbial loop. Ammonium measured in parallel with other nutrients is crucial in understanding the small temporal scale changes in oceanographic ecology. Despite the importance of measuring ammonium at sea, owing to its lability, there is no consensus on the best method. The lack of availability of certified reference materials for ammonium in seawater also makes it difficult to assess the accuracy and reproducibility of ammonium measurements. In this study we present a modified segmented flow analysis method using ortho-phthaldialdehyde (OPA) with fluorescence detection to measure ammonium at sea together with four other macro-nutrients (nitrate, nitrite, silicate and phosphate) in near real time. An in-house ammonium quality control (QC) material was produced to improve the accuracy and repeatability of the measurement at sea. The QC was prepared following two different methods and s.....
JournalFrontiers in Marine Science
Sustainable Development Goals (SDG)14.A
Essential Ocean Variables (EOV)Nutrients
Maturity LevelTRL 9 Actual system "mission proven" through successful mission operations (ground or space)
Best Practice TypeManual (incl. handbook, guide, cookbook etc)
Standard Operating Procedure
CitationRees, C., Janssens, J., Sherrin, K., Hughes, P., Tibbe, S., McMahon, M., McDonald, J., Camac, A., Schwanger, C. and Marouchos, A. (2021) Method for Reproducible Shipboard Segmented Flow Analysis Ammonium Measurement Using an In-House Reference Material for Quality Control. Frontiers in Marine Science, 8:581901,15pp. DOI: 10.3389/fmars.2021.581901
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