Imaging Flow Cytometry Protocols for Examining Phagocytosis of Microplastics and Bioparticles by Immune Cells of Aquatic Animals.
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Date
2019Author
Park, Y.
Abihssira-García, I.S.
Thalmann, S.
Wiegertjes, G.F.
Barreda, D.R.
Olsvik, P.A.
Kiron, V.
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Show full item recordAbstract
Imaging flow cytometry (IFC) is a powerful tool which combines flow cytometry with digital
microscopy to generate quantitative high-throughput imaging data. Despite various
advantages of IFC over standard flow cytometry, widespread adoption of this technology
for studies in aquatic sciences is limited, probably due to the relatively high equipment
cost, complexity of image analysis-based data interpretation and lack of core facilities
with trained personnel. Here, we describe the application of IFC to examine phagocytosis
of particles including microplastics by cells from aquatic animals. For this purpose, we
studied (1) live/dead cell assays and identification of cell types, (2) phagocytosis of
degradable and non-degradable particles by Atlantic salmon head kidney cells and (3)
the effect of incubation temperature on phagocytosis of degradable particles in three
aquatic animals–Atlantic salmon, Nile tilapia, and blue mussel. The usefulness of the
developed method was assess.....
Journal
Frontiers in ImmunologyVolume
11Issue
Article 203Page Range
11pp.Document Language
enSustainable Development Goals (SDG)
14.AMaturity Level
TRL 8 Actual system completed and "mission qualified" through test and demonstration in an operational environment (ground or space)Best Practice Type
Manual (incl. handbook, guide, cookbook etc)DOI Original
https://doi.org/10.3389/fimmu.2020.00203Citation
Park, Y., Abihssira-García, I.S., Thalmann, S., Wiegertjes, G.F., Barreda, D.R., Olsvik, P.A. and Kiron, V. (2020) Imaging Flow Cytometry Protocols for Examining Phagocytosis of Microplastics and Bioparticles by Immune Cells of Aquatic Animals. Frontiers in Immunology, 11:203, 11pp. DOI: 10.3389/fimmu.2020.00203Collections
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