On-site sequencing speeds up and re-directs field-based microbiology.
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As a field-going molecular microbial ecologist, I often experience an extreme high feeling or bout of excitement during immersion in my study environment. However, when I return to my laboratory, generally in the cold and dark winter months, I ‘crash’ or reach a low point, due to my realization of how many samples we obtained in the field and the extent of processing, sequencing and analysis time that we still need to devote to the samples before we can draw any conclusions and share our findings. In an effort to lessen my now regular post-field crashes, I have started following the development of mobile sequencing technology. Oxford Nanopore Technologies has recently made large strides in this area, producing the MinION a hand-held flow-cell costing $1,000 that can connect to a laptop computer. The company is developing the even more mobile SmidgION, a lower power device that will run on a smartphone. Depending on the type of MinION sequencing conducted (amplicon, m.....
JournalEnvironmental Microbiology Reports
Sustainable Development Goals (SDG)14
Best Practice TypeManual (incl. handbook, guide, cookbook etc)
CitationApprill, A. (2019) On‐site sequencing speeds up and re‐directs field‐based microbiology. Environmental Microbiology Reports, 11, pp.45-47. DOI: https://doi.org/10.1111/1758-2229.12732