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dc.contributor.authorHoward, Meredith D.A.
dc.contributor.authorKudela, Raphael
dc.contributor.authorCaron, David
dc.contributor.authorSmith, Jayme
dc.contributor.authorHayashi, Kendra
dc.identifier.citationHoward, M.D.A.; Hayashi, K.; Smith, J.; Kudela, R. and Caron, D. (2018) Standard Operating Procedure for Solid Phase Adsorption Toxin Testing (SPATT) Assemblage and Extraction of HAB Toxins. University of California and University of Southern California, 14pp. DOI:
dc.description.abstractSPATT has been developed and tested for a variety of resins (see review by Kudela, 2017). These Standard Operating Procedures (SOP) outline the use of the resin DIAON HP20 since it has been demonstrated to quantify microcystins, anatoxin-a, saxitoxin, domoic acid, and okadaic acid in fresh, brackish, and marine waters, all from the same SPATT sampling device (Lane et al. 2010; Miller et al. 2010, Kudela 2011, Gibble and Kudela, 2014, Howard et al., 2017, Kudela, 2017, Peacock et al., 2018). Cylindrospermopsin and nodularin have also been detected using SPATT with DIAON HP20 resin in California, however, the use of DIAON HP20 for these toxins has not been well characterized in the laboratory. This methodology is ready for wider adoption by the research, monitoring, and management communities interested in detecting and tracking the dynamics of freshwater and marine toxins. The SOP described herein for the assemblage and construction of SPATT bags can be used for deployment in freshwater, brackish and marine environments and the extraction process described below is for the analysis of both marine toxins and cyanotoxins.en_US
dc.description.sponsorshipFunding for this study was provided by National Oceanic and Atmospheric Administration Monitoring and Event Response for Harmful Algal Bloom (MERHAB) grant NA15NOS4780204 awarded to Meredith D.A. Howard, Raphael Kudela and David A. Caron and ECOHAB grants NA11NOS4780052 and subaward NA08OAR4320894 (DC) and NA11NOS4780030 (RMK).en_US
dc.publisherUniversity of California and University of Southern Californiaen_US
dc.rightsCC0 1.0 Universal*
dc.subject.otherHarmful Algal Bloomsen_US
dc.subject.otherSolid Phase Adsorption Toxin Tracking (SPATT)en_US
dc.titleStandard Operating Procedure for Solid Phase Adsorption Toxin Testing (SPATT) Assemblage and Extraction of HAB Toxins.en_US
dc.publisher.placeSanta Cruz, CAen_US
dc.subject.parameterDisciplineParameter Discipline::Chemical oceanography::Other organic chemical measurementsen_US
dc.subject.parameterDisciplineParameter Discipline::Biological oceanography::Macroalgae and seagrassen_US
dc.description.maturitylevelTRL 7 System prototyping demonstration in an operational environment (ground or space)en_US
dc.description.bptypeStandard Operating Procedureen_US Kudela Howard

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CC0 1.0 Universal
Except where otherwise noted, this item's license is described as CC0 1.0 Universal