Quantifying pCO2 in biological ocean acidification experiments: a comparison of four methods.
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Fabricius, Katharina E.
Munday, Philip L.
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Quantifying the amount of carbon dioxide (CO2) in seawater is an essential component of ocean acidification research; however, equipment for measuring CO2 directly can be costly and involve complex, bulky apparatus. Consequently, other parameters of the carbonate system, such as pH and total alkalinity (AT), are often measured and used to calculate the partial pressure of CO2 (pCO2) in seawater, especially in biological CO2-manipulation studies, including large ecological experiments and those conducted at field sites. Here we compare four methods of pCO2 determination that have been used in biological ocean acidification experiments: 1) Versatile INstrument for the Determination of Total inorganic carbon and titration Alkalinity (VINDTA) measurement of dissolved inorganic carbon (CT) and AT, 2) spectrophotometric measurement of pHT and AT, 3) electrode measurement of pHNBS and AT, and 4) the direct measurement of CO2 using a portable CO2 equilibrator with a non-dispersive i.....
Sustainable Development Goals (SDG)14.3
Essential Ocean Variables (EOV)Inorganic carbon
Maturity LevelTRL 8 Actual system completed and "mission qualified" through test and demonstration in an operational environment (ground or space)
Best Practice TypeManual (incl. handbook, guide, cookbook etc)
Standard Operating Procedure
CitationWatson, S-A.; Fabricius, K.E. and Munday, P.L. (2017) Quantifying pCO2 in biological ocean acidification experiments: A comparison of four methods. PLoS ONE 12 e0185469, 16pp. DOI: https://doi. org/10.1371/journal.pone.0185469
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