dc.contributor.author | Aylagas, Eva | |
dc.contributor.author | Mendibil, Iñaki | |
dc.contributor.author | Borja, Ángel | |
dc.contributor.author | Rodríguez-Ezpeleta, Naiara | |
dc.date.accessioned | 2019-08-09T23:31:10Z | |
dc.date.available | 2019-08-09T23:31:10Z | |
dc.date.issued | 2016 | |
dc.identifier.citation | Aylagas, E,; Mendibil, I.; Borja, Á. and
Rodríguez-Ezpeleta, N. (2016) Marine
Sediment Sample Pre-processing for
Macroinvertebrates Metabarcoding:
Mechanical Enrichment and
Homogenization.
Frontiers in Marine Science, 3:203, 8pp DOI:10.3389/fmars.2016.00203 | en_US |
dc.identifier.uri | http://hdl.handle.net/11329/1016 | |
dc.identifier.uri | http://dx.doi.org/10.25607/OBP-548 | |
dc.description.abstract | Metabarcoding is an accurate and cost-effective technique that allows for simultaneous
taxonomic identification of multiple environmental samples. Application of this technique
to marine benthic macroinvertebrate biodiversity assessment for biomonitoring purposes
requires standardization of laboratory and data analysis procedures. In this context,
protocols for creation and sequencing of amplicon libraries and their related
bioinformatics analysis have been recently published. However, a standardized protocol
describing all previous steps (i.e., processing and manipulation of environmental
samples for macroinvertebrate community characterization) is lacking. Here, we
provide detailed procedures for benthic environmental sample collection, processing,
enrichment for macroinvertebrates, homogenization, and subsequent DNA extraction
for metabarcoding analysis. Since this is the first protocol of this kind, it should be of use
to any researcher in this field, having the potential for improvement. | en_US |
dc.language.iso | en | en_US |
dc.rights | Attribution 4.0 International | * |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | * |
dc.subject.other | Environmental samples | en_US |
dc.subject.other | Laboratory procedures | en_US |
dc.subject.other | Sample manipulation | en_US |
dc.subject.other | DNA | en_US |
dc.subject.other | Biomonitoring | en_US |
dc.title | Marine Sediment Sample Pre-processing for Macroinvertebrates Metabarcoding: Mechanical Enrichment and Homogenization. | en_US |
dc.type | Journal Contribution | en_US |
dc.description.refereed | Refereed | en_US |
dc.format.pagerange | 8pp. | en_US |
dc.identifier.doi | 10.3389/fmars.2016.00203 | |
dc.subject.parameterDiscipline | Parameter Discipline::Biological oceanography::Other biological measurements | en_US |
dc.bibliographicCitation.title | Frontiers in Marine Science | en_US |
dc.bibliographicCitation.volume | 3 | en_US |
dc.bibliographicCitation.issue | Article 203 | en_US |
dc.description.bptype | Guide | en_US |
dc.description.frontiers | 2016-06-15 | |
obps.contact.contactname | Eva Aylagas | |
obps.contact.contactemail | eaylagas@azti.es | |
obps.resourceurl.publisher | https://www.frontiersin.org/articles/10.3389/fmars.2016.00203/full | en_US |