TARA Microbiomes Procedures
https://repository.oceanbestpractices.org/handle/11329/1794
2024-03-29T06:40:55ZExpanding "Tara" Oceans Protocols for Underway, Ecosystemic Sampling of the Ocean-Atmosphere Interface During Tara Pacific Expedition (2016–2018).
https://repository.oceanbestpractices.org/handle/11329/1439
Expanding "Tara" Oceans Protocols for Underway, Ecosystemic Sampling of the Ocean-Atmosphere Interface During Tara Pacific Expedition (2016–2018).
Gorsky, Gabriel; Bourdin, Guillaume; Lombard, Fabien; Pedrotti, Maria Luiza; Audrain, Samuel; Bin, Nicolas; Boss, Emmanuel; Bowler, Chris; Cassar, Nicolas; Caudan, Loic; Chabot, Genevieve; Cohen, Natalie R.; Cron, Daniel; De Vargas, Colomban; Dolan, John R.; Douville, Eric; Elineau, Amanda; Flores, J. Michel; Ghiglione, Jean Francois; Haëntjens, Nils; Hertau, Martin; John, Seth G.; Kelly, Rachel L.; Koren, Ilan; Lin, Yajuan; Marie, Dominique; Moulin, Clémentine; Moucherie, Yohann; Pesant, Stéphane; Picheral, Marc; Poulain, Julie; Pujo-Pay, Mireille; Reverdin, Gilles; Romac, Sarah; Sullivan, Mathew B.; Trainic, Miri; Tressol, Marc; Troublé, Romain; Vardi, Assaf; Voolstra, Christian R.; Wincker, Patrick; Agostini, Sylvain; Banaigs, Bernard; Boissin, Emilie; Forcioli, Didier; Furla, Paola; Galand, Pierre E.; Gilson, Eric; Reynaud, Stéphanie; Sunagawa, Shinichi; Thomas, Olivier P.; Thurber, Rebecca Lisette Vega; Zoccola, Didier; Planes, Serge; Allemand, Denis; Karsenti, Eric
Interactions between the ocean and the atmosphere occur at the air-sea interface
through the transfer of momentum, heat, gases and particulate matter, and through
the impact of the upper-ocean biology on the composition and radiative properties of
this boundary layer. The Tara Pacific expedition, launched in May 2016 aboard the
schooner Tara, was a 29-month exploration with the dual goals to study the ecology
of reef ecosystems along ecological gradients in the Pacific Ocean and to assess
inter-island and open ocean surface plankton and neuston community structures. In
addition, key atmospheric properties were measured to study links between the two
boundary layer properties. A major challenge for the open ocean sampling was the lack
of ship-time available for work at “stations”. The time constraint led us to develop new
underway sampling approaches to optimize physical, chemical, optical, and genomic
methods to capture the entire community structure of the surface layers, from viruses
to metazoans in their oceanographic and atmospheric physicochemical context. An
international scientific consortium was put together to analyze the samples, generate
data, and develop datasets in coherence with the existing Tara Oceans database.
Beyond adapting the extensive Tara Oceans sampling protocols for high-resolution
underway sampling, the key novelties compared to Tara Oceans’ global assessment
of plankton include the measurement of (i) surface plankton and neuston biogeography
and functional diversity; (ii) bioactive trace metals distribution at the ocean surface and
metal-dependent ecosystem structures; (iii) marine aerosols, including biological entities;
(iv) geography, nature and colonization of microplastic; and (v) high-resolution underway
assessment of net community production via equilibrator inlet mass spectrometry. We
are committed to share the data collected during this expedition, making it an important
resource important resource to address a variety of scientific questions.
2019-01-01T00:00:00Z